Journal: Cell metabolism

Article Title: Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue

doi: 10.1016/j.cmet.2022.02.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD86 Antibody, anti-mouse, FITC, Miltenyi Biotec , Miltenyi Biotec , Cat# 130–123-672; RRID:AB_2889633.

Techniques: Immunofluorescence, Recombinant, Cytometry, Purification, shRNA, Cell Culture, Lysis, XF Assay, Staining, Isolation, Transfection, Live Cell Imaging, Mouse Assay, Software, Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: A Comparative Study of the Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stem Cells (MSCs) Efficiency on Generating MSC-Educated Macrophages (MEMs)

doi: 10.31557/APJCP.2022.23.9.3083

Figure Lengend Snippet: Antibodies Used to Analyze Surface Markers of MQs

Article Snippet: CD86 , FITC , Miltenyi Biotech, Germany , M1 MQ.

Techniques:

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: A Comparative Study of the Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stem Cells (MSCs) Efficiency on Generating MSC-Educated Macrophages (MEMs)

doi: 10.31557/APJCP.2022.23.9.3083

Figure Lengend Snippet: Comparison of MQ CD Markers on Day 5, 7 and 10. Monocytes on day +5 express high level of CD11b/CD68 while expressing low level of CD80, CD86, CD206 and CD163. High expression of CD11b/CD68 indicated the monocyte differentiation to MQs during in vitro culture. Low expression of polarization markers also indicated a lack of differentiation into inflammatory/anti-inflammatory phenotype and, in fact, remaining the state of resting (M0) MQs. (A) Unstained cells; (B) Cells expressing CD11b/CD68; (C) Cells expressing CD80/CD86; (D) Cells expressing CD206/CD163. PE, phycoerythrin; FITC, fluorescein isothiocyanate

Article Snippet: CD86 , FITC , Miltenyi Biotech, Germany , M1 MQ.

Techniques: Comparison, Expressing, In Vitro

Journal: Nucleic Acids Research

Article Title: Epstein–Barr virus-mediated transformation of B cells induces global chromatin changes independent to the acquisition of proliferation

doi: 10.1093/nar/gkt886

Figure Lengend Snippet: Comparison of histone modification changes during transformation of RBLs with mutant forms of EBV and IL-4/CD40L-stimulated cells. ( A ) Comparison of activation and proliferation levels between B cells infected with wild type EBV (WT EBV), the LMP-1 and EBNA-2 deficient forms of EBV (LMP1 KO and EBNA2 KO), and cells stimulated with IL-4/CD40L (ACT). The left panel shows the proportion of activated cells (left; determined by FACS analysis with CD86) and the right panel the proportion of dividing cells (determined with tritiated thymidine). ( B ) Quantitation of levels of infection with LMP1-expressing lentivirus measured by GFP fluorescence and FACS analysis. ( C ) Analysis by western blot of a time course experiment with antibodies against H3K4me3, H3K9me3, H3K27me3, total H3, H4K20me3 and total H4 of RBLs infected with wild type EBV, EBV deficient for LMP-1, EBV deficient for EBNA-2, IL-4/CD40L-stimulated B cells and IL-4/CD40L-stimulated B cells infected with LMP-1. ( D ) Densitometric quantitation of western blot data. The average of three independent experiments is represented. Data are presented relative to levels in RBL. ( E ) Top panel, XIC of isobaric H3 peptides from different B-cell populations. Differentially modified peptides were chromatographically separated and individual peaks identified by tandem MS spectra. Relative levels of individual isoforms were quantified using the Genesis peak detection module of the Xcalibur software package (Thermo Fisher Scientific). X -axis represents retention time, y -axis the intensity of ion currents in the Orbitrap mass spectrometer. Bottom panel, bar chart of H3K27me3 levels relative to the two other modification states of this peptide. Error bars represent the SD of two independent biological replicates. Abbreviations : unmod = unmodified peptide, me1 = single mono-methylated lysine. ( F ) Quantitative ChIP assays showing changes in H4K20me3, H3K27me3 and H3K9me3 between RBLs and B cells infected wild type EBV, EBV deficient for LMP-1, EBV deficient for EBNA-2, IL-4/CD40L-stimulated B cells and IL-4/CD40L-stimulated B cells infected with LMP-1 (LMP1 LV). The sequences correspond to promoter gene sequences from the ChIP-seq analysis. GAPDH was used as a control sequence.

Article Snippet: EBV-infected or CD40L/IL-4-activated B cells were harvested and stained for CD86 expression with an anti-human CD86 FITC-conjugated (EuroBioSciences) at 1:100 concentration.

Techniques: Comparison, Modification, Transformation Assay, Mutagenesis, Activation Assay, Infection, Quantitation Assay, Expressing, Fluorescence, Western Blot, Software, Mass Spectrometry, Methylation, ChIP-sequencing, Control, Sequencing